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The high voltage insulation performance of electric vehicles is related to the safety of the equipment and personnel. The measurement method of insulation resistance has disadvantages such as low detection accuracy, no online measurement, and weak anti-interference ability. In-depth on the basis of traditional insulation resistance measurement is presented in this paper, combining with the characteristics of the electric car high pressure system, puts forward a kind of low frequency low pressure injection insulation resistance testing method, solves the unable to online real-time detection, positive and negative bus for car body fall at the same time you can’t measure insulation resistance, etc. The experimental results show that the method can detect the insulation performance of the high voltage system in real time and the error is within 5%.